Establishment of Embryonic Stem Cell-like Cells in Pigs
نویسنده
چکیده
We generated unique porcine embryonic stem cell-like cells (pESLCs) by using inhibitors of GSK3β and MEK1. The pESLCs colonies are dome-shape morphology and show the expression of undifferentiated markers including OCT4 and NANOG. Moreover, the self-renewal capacity and morphology of the cell lines are LIFdependent, consistent with the LIF receptors expression and STAT3 phosphorylation, and continuous culture for over 100 passages was possible without any overt morphological changes and telomerase activity.
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